Regulatory

Part:BBa_K625003:Design

Designed by: Michael Eichenberger   Group: iGEM11_ETH_Zurich   (2011-09-21)


Pu promoter short version


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All necessary (Valls et al, 2003) elements of the promoter were kept, whereas the first part of XylU and its RBS were deleted.


Source

PCR from the plasmid pCK04AxylR provided by Prof Sven Panke

References